gerKD [2019-04-22 18:34:17]

negative effector (D protein) of the GerKA-GerKB-GerKC germinant receptor

locus: BSU03690

pI: 10.01

mw: 8.25 kDa

protein length: 73 aa

gene length: 222 bp

function: germination

product: negative effector of the GerKA-GerKB-GerKC germinant receptor

essential: no

synonyms: yczF

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.1|Germinant receptors]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 419,763 → 419,984

Phenotypes of a mutant

• faster [SW|germination] with the AGFK germinant mixture [Pubmed|23625846]
• higher sensitivity to AGFK germinants ([SW|germination] at lower concentrations) [Pubmed|23625846]

The protein

[SW|Localization]

• membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|DBC4A2711F5079AF1C1B743DA8D7A813DAA0827F|gerKD]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|23625846], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • expressed during [SW|sporulation] in the forespore ([protein|search|SigG]) [Pubmed|23625846]

Biological materials

Mutant

• BKE03690 (Δ[gene|DBC4A2711F5079AF1C1B743DA8D7A813DAA0827F|gerKD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTCATCATCCTCCGC, downstream forward: _UP4_AACCCCTAAAGGGGGGCGCT
• BKK03690 (Δ[gene|DBC4A2711F5079AF1C1B743DA8D7A813DAA0827F|gerKD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTCATCATCCTCCGC, downstream forward: _UP4_AACCCCTAAAGGGGGGCGCT

References

• 23625846,22383849,27766092